Bioprocessing Tutorial, Nov 1, 2015 (Vol. 35, No. 19),
Novel Method for Glycoprotein Expression
Utilizing CAP-Go Cells for Optimizing Serum Half-Life and Glycosylation
Silke Wissing, Ph.D., Jens Wolfel, Ph.D., Helmut Kewes, Christian Niehus, Corinna Bialek, Sabine Hertel, Nicole Faust, Ph.D.
(Cevec Pharmaceuticals)
- rhC1 Inh expressed from CAP-Go.2 cells that are optimized for the expression of N- and O-linked glycosylated proteins display glycan patterns closely similar to plasma-derived C1 Inh. The resulting molecule has a significantly prolonged serum half-life as compared to its counterpart generated on a conventional human cell line.
- Our new recombinant molecule matches serum-derived C1 Inh in all the aspects analyzed: specific activity, serum half-life, and glycosylation patterns. Additionally, CAP-Go.2 cells offer the advantage of being producible at large scales on a safe platform. Because the cell line is compatible with high-titer, scalable processing, it could produce C1 Inh in relative abundance, a market supply sufficient even for additional treatments, which so far cannot be served by plasma-derived products due to limited access.