..Although earlier studies described the production of human recombinant PLTP (rhPLTP) in eukaryotic cells9,10,11, only low levels of active rhPLTP could be produced in bioreactors, thus hampering the possibility of using it at therapeutic levels in sepsis. Importantly, PLTP is a complex glycosylated protein, with six distinct N-glycosylation sites which are essential for normal activity12, 13. In earlier studies, transgenic rabbits successfully produced recombinant human proteins14,15,16,17, some of which are currently under investigation in human therapeutics (alpha-glucosidase18, C1-esterase inhibitor19). The production of recombinant proteins by rabbits offers a number of advantages: easy generation of transgenic founders and offspring, high fertility, post-translational modifications close to what occurs in humans, insensitivity to prion diseases, and a low risk of transmission of severe diseases to humans.
goed onthouden..